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Description:The biological mechanisms underlying the association warrant further clarification. Iris Color Grading The detailed grading protocol of iris color has been described in our previous report on the association between iris color and refractive error. In total, grade 7 students, from 10 middle schools, aged 13 to 14 years in Mojiang located in Southwestern China were included in the analysis. Participants were seated and their heads were stabilized using a chin rest and brow bar when measurements were performed. The higher grade was assigned if a photo was considered to be between two consecutive grades. A grading system was developed to assess iris color based on standardized slit-lamp photographs.

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